Fluorescence Pattern Analysis-How To Achieve
نویسنده
چکیده
In the rapidly expanding fields of cellular and molecular biology, fluorescence illumination and observation is becoming one of the techniques of choice to study the localization and dynamics of proteins, organelles, and other cellular compartments, as well as a tracer of intracellular protein trafficking. Quantitative imaging of fluorescent proteins and patterns is accomplished with a variety of techniques, including wide-field, confocal and multiphoton microscopy, ultrafast low-light level digital cameras and multi tracking laser control systems. These microscopic images can be of 2-dimensional or 3-dimensional nature, or even videos recording the life cycle of a cell.
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